免费真人视频app,99久久国产露脸精品竹菊传媒,国产麻传媒精品国产av,麻豆人妻少妇精品无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

簡要描述:HTB-26 MDA-MB-231 人乳腺癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-05-25
  • 訪  問  量:2659

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

ATCC® Number: HTB-26™ Price: $256.00

Designations: MDA-MB-231

Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


HTB-26 MDA-MB-231 人乳腺癌細(xì)胞

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: epidermal growth factor (EGF), expressed

transforming growth factor alpha (TGF alpha), expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,13

D13S317: 13

D16S539: 12

D5S818: 12

D7S820: 8,9

THO1: 7,9.3

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: The cell line is aneuploid female (modal number = 64, range = 52 to 68), with chromosome counts in the near-triploid range. Normal chromosomes N8 and N15 were absent. Eleven stable rearranged marker chromosomes are noted as well as unassignable chromosomes in addition to the majority of autosomes that are trisomic. Many of the marker chromosomes are identical to those shown in the karyotype reported by K.L. Satya-Prakash, et al.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1-2

PGM1, 1-2

PGM3, 1

Age: 51 years adult

Gender: female

Ethnicity: Caucasian

Comments: The cells express the WNT7B oncogene [PubMed: 8168088].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C without CO2.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

purified DNA:ATCC 45518

purified DNA:ATCC 45519

purified DNA:ATCC HTB-26D

purified RNA:ATCC HTB-26R

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

22977: Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

23010: Dickstein B, et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993. PubMed: 8408230

23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088

26321: Satya-Prakash KL, et al. Cytogenetic analysis on eight human breast tumor cell lines: high frequencies of 1q, 11q and HeLa-like marker chromosomes. Cancer Genet. Cytogenet. 3: 61-73, 1981. PubMed: 7272986

32272: Katayose Y, et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997. PubMed: 9407946

32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

32341: Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194

32489: De Vincenzo R, et al. Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anticancer Drug Des. 13: 19-33, 1998. PubMed: 9474240

33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
无码国产色欲xxxx视频| 男人用嘴添女人下身免费视频| 韩国午夜理伦三级在线观看中文版| 第一次进入女朋友的身体注意事项| 真人做人试看60分钟免费视频| 国精品人妻无码一区二区三区软件| 老卫抱着淑蓉进房间第八集视频| 97精产国品一二三产区| 《与上司出轨的人妻》| 插插网| 男女做爰猛烈叫床视频动态图| 未满十八18禁止免费无码网站| 亚洲国产精品自在自线观看| 级毛片内射视频| 香港60部三级未删版电影| 丁香婷婷综合激情五月色| eeuss影院www在线观看| 欧美人与性动交α欧美精品| 军人的粗大h拔不出来| 熟透的岳跟岳弄了69视频| 乔家的儿女电视剧全集免费播放| 狠狠色丁香婷婷综合久久97| 18禁裸乳无遮挡啪啪无码免费| 亚洲欧美强伦一区二区| 曰批国产精品视频免费观看| 少爷托着娇乳撞击娇吟| 欧美性xxxx极品少妇| 高中陪读房间的呻吟声| 黄色视频app下载| 久久精品人妻少妇一区二区三区| 女人19水真多毛片学生| 人人妻人人玩人人澡人人爽| 岳的又肥又大又紧水有多视频| 女人荫蒂添的好舒服a片| 巨胸爆乳美女露双奶头挤奶| 丰满人妻在公车被猛烈进入电影| 丰满老熟妇好大bbbbb| 日产乱码一二三区别免费| 久久久国产精品免费a片分环卫| 免费观看电视剧全集在线播放| 国产精品99久久久久久|